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pcdna3 blm  (Addgene inc)


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    Structured Review

    Addgene inc pcdna3 blm
    Pcdna3 Blm, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+blm/pcDNA3%2FBLM+(Plasmid+%23111766)/pmc13036488-42-54-55
    Average 92 stars, based on 2 article reviews
    pcdna3 blm - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: TRF2–RAP1 represses RAD51-dependent homology-directed telomere repair by promoting BLM-mediated D-loop unwinding and inhibiting BLM–DNA2-dependent 5′-end resection
    Article Snippet: .. Human BLM cDNA with a C-terminal Flag-tag from pcDNA3/BLM (Addgene #111 766) was subcloned into phCMV1-2 × MBP-MCS vector ( ). ..

    Article Title: TRF2-RAP1 represses RAD51-dependent homology-directed telomere repair by promoting BLM-mediated D-loop unwinding and inhibiting BLM-DNA2-dependent 5'-end resection.
    Article Snippet: .. Human BLM cDNA with a C-terminal Flag-tag from pcDNA3 / BLM (Addgene #111 766) was subcloned into phCMV1-2 × MBP-MCS vector ( 32 ). ..

    Transformation Assay:

    Article Title: TRF2-RAP1 inhibits homology-directed repair of telomeres by promoting BLM-mediated removal of telomere R-loops.
    Article Snippet: .. hRAP1 complementary DNA (cDNA) with a C-terminal Flagtag was subcloned into the BglII/HindIII site of pTric-HisB vector. hTRF2 (Addgene #50488) or hRAP1 in pTricHisB vectors was transformed into Esc heric hia coli BL21(DE3) cells and purified by Ni Sepharose and anti-FLAG M2 affinity chromatography as described [ 19 ]. hBLM cDNA with a Cterminal Flag tag from pcDNA3/BLM (Addgene #111766) was subcloned into phCMV1-2 × MBP-MCS vector [ 19 , 34 ]. .. TRF2 and BLM mutations were generated with a site-directed mutagenesis kit (Agilent).

    Article Title: TRF2–RAP1 inhibits homology-directed repair of telomeres by promoting BLM-mediated removal of telomere R-loops
    Article Snippet: .. hRAP1 complementary DNA (cDNA) with a C-terminal Flag-tag was subcloned into the BglII/HindIII site of pTric-HisB vector. hTRF2 (Addgene #50488) or hRAP1 in pTricHisB vectors was transformed into Escherichia coli BL21(DE3) cells and purified by Ni Sepharose and anti-FLAG M2 affinity chromatography as described [ ]. hBLM cDNA with a C-terminal Flag tag from pcDNA3/BLM (Addgene #111766) was subcloned into phCMV1-2 × MBP-MCS vector [ , ]. .. TRF2 and BLM mutations were generated with a site-directed mutagenesis kit (Agilent).

    Purification:

    Article Title: TRF2-RAP1 inhibits homology-directed repair of telomeres by promoting BLM-mediated removal of telomere R-loops.
    Article Snippet: .. hRAP1 complementary DNA (cDNA) with a C-terminal Flagtag was subcloned into the BglII/HindIII site of pTric-HisB vector. hTRF2 (Addgene #50488) or hRAP1 in pTricHisB vectors was transformed into Esc heric hia coli BL21(DE3) cells and purified by Ni Sepharose and anti-FLAG M2 affinity chromatography as described [ 19 ]. hBLM cDNA with a Cterminal Flag tag from pcDNA3/BLM (Addgene #111766) was subcloned into phCMV1-2 × MBP-MCS vector [ 19 , 34 ]. .. TRF2 and BLM mutations were generated with a site-directed mutagenesis kit (Agilent).

    Article Title: TRF2–RAP1 inhibits homology-directed repair of telomeres by promoting BLM-mediated removal of telomere R-loops
    Article Snippet: .. hRAP1 complementary DNA (cDNA) with a C-terminal Flag-tag was subcloned into the BglII/HindIII site of pTric-HisB vector. hTRF2 (Addgene #50488) or hRAP1 in pTricHisB vectors was transformed into Escherichia coli BL21(DE3) cells and purified by Ni Sepharose and anti-FLAG M2 affinity chromatography as described [ ]. hBLM cDNA with a C-terminal Flag tag from pcDNA3/BLM (Addgene #111766) was subcloned into phCMV1-2 × MBP-MCS vector [ , ]. .. TRF2 and BLM mutations were generated with a site-directed mutagenesis kit (Agilent).

    Affinity Chromatography:

    Article Title: TRF2-RAP1 inhibits homology-directed repair of telomeres by promoting BLM-mediated removal of telomere R-loops.
    Article Snippet: .. hRAP1 complementary DNA (cDNA) with a C-terminal Flagtag was subcloned into the BglII/HindIII site of pTric-HisB vector. hTRF2 (Addgene #50488) or hRAP1 in pTricHisB vectors was transformed into Esc heric hia coli BL21(DE3) cells and purified by Ni Sepharose and anti-FLAG M2 affinity chromatography as described [ 19 ]. hBLM cDNA with a Cterminal Flag tag from pcDNA3/BLM (Addgene #111766) was subcloned into phCMV1-2 × MBP-MCS vector [ 19 , 34 ]. .. TRF2 and BLM mutations were generated with a site-directed mutagenesis kit (Agilent).

    Article Title: TRF2–RAP1 inhibits homology-directed repair of telomeres by promoting BLM-mediated removal of telomere R-loops
    Article Snippet: .. hRAP1 complementary DNA (cDNA) with a C-terminal Flag-tag was subcloned into the BglII/HindIII site of pTric-HisB vector. hTRF2 (Addgene #50488) or hRAP1 in pTricHisB vectors was transformed into Escherichia coli BL21(DE3) cells and purified by Ni Sepharose and anti-FLAG M2 affinity chromatography as described [ ]. hBLM cDNA with a C-terminal Flag tag from pcDNA3/BLM (Addgene #111766) was subcloned into phCMV1-2 × MBP-MCS vector [ , ]. .. TRF2 and BLM mutations were generated with a site-directed mutagenesis kit (Agilent).

    FLAG-tag:

    Article Title: TRF2-RAP1 inhibits homology-directed repair of telomeres by promoting BLM-mediated removal of telomere R-loops.
    Article Snippet: .. hRAP1 complementary DNA (cDNA) with a C-terminal Flagtag was subcloned into the BglII/HindIII site of pTric-HisB vector. hTRF2 (Addgene #50488) or hRAP1 in pTricHisB vectors was transformed into Esc heric hia coli BL21(DE3) cells and purified by Ni Sepharose and anti-FLAG M2 affinity chromatography as described [ 19 ]. hBLM cDNA with a Cterminal Flag tag from pcDNA3/BLM (Addgene #111766) was subcloned into phCMV1-2 × MBP-MCS vector [ 19 , 34 ]. .. TRF2 and BLM mutations were generated with a site-directed mutagenesis kit (Agilent).

    Article Title: TRF2–RAP1 inhibits homology-directed repair of telomeres by promoting BLM-mediated removal of telomere R-loops
    Article Snippet: .. hRAP1 complementary DNA (cDNA) with a C-terminal Flag-tag was subcloned into the BglII/HindIII site of pTric-HisB vector. hTRF2 (Addgene #50488) or hRAP1 in pTricHisB vectors was transformed into Escherichia coli BL21(DE3) cells and purified by Ni Sepharose and anti-FLAG M2 affinity chromatography as described [ ]. hBLM cDNA with a C-terminal Flag tag from pcDNA3/BLM (Addgene #111766) was subcloned into phCMV1-2 × MBP-MCS vector [ , ]. .. TRF2 and BLM mutations were generated with a site-directed mutagenesis kit (Agilent).



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